Synergistic Extraction of Totalflavonoids from Pomegranate Peel in Panzhihua by Joint Use of Cellulase and Ultrasonic Wave

To study the synergistic extraction of flavonoids in Panzhihua pomegranate peel by ultrasonic-assisted cellulase method, the effects of ethanol volume fraction, solid-liquid ratio, enzymatic activity, ultrasonic power, extraction time and extraction temperature on the yield rate of total flavonoids are examined.By orthogonal experiment design, the optimal extraction conditions were acquired:when the enzyme activity is 20 U/mL, ultrasonic power is at 280 W, extraction time lasts 20 minutes, extraction temperature at 60℃, the yield rate of total flavonoids could reach 8.805%.The scavenging ability of extract for 1, 1-diphenyl-2-picrylhydrazyl free radical was stronger than vitamin C and rutin and the scavenging ability was of good dose-effect relationship.The experiment method can serve as a good means for the better utilization of Panzhihua pomegranate peel resource.